《植物生理学报》 2015, 51(10): 1611-1616

NaCl胁迫下宁夏不同地区沙枣种子萌发特性的比较研究

林静¹, 刘艳¹, 李金胜², 刘晓娟¹, 王宇鹏¹, 王宝山¹, 陈敏^1,*

¹山东师范大学生命科学学院, 逆境植物重点实验室, 济南250014; ²山东省东营菁华现代林业发展有限公司, 山东东营257000

通信作者：陈敏；E-mail: chenminrundong@126.com；Tel: 0531-86180745

摘　要：

对从宁夏平罗、中宁两个地区收集的当年生沙枣种子, 分别用含有不同浓度NaCl (0、0.3%、0.6%、0.9%和1.2%, m/V)的1/5 (V/V) Hoagland溶液处理, 以探讨这两个地区沙枣种子在萌发阶段对盐胁迫的响应特性及可能原因。结果表明, 随着NaCl浓度的升高, 中宁和平罗沙枣种子的萌发率均逐渐下降, 与中宁的沙枣种子相比, 平罗的沙枣种子表现出更强的抗盐能力。伊文斯蓝(Evans blue)染色和质膜透性实验结果表明, 随着NaCl浓度的增加, 中宁和平罗沙枣的种子胚的细胞膜透性逐渐增加, 即NaCl胁迫破坏了种子胚的质膜选择透性, 使其增大, 细胞内大量电解质外渗, 破坏了细胞的稳态。通过LiCl溶液和等渗的甘露醇溶液处理两个地区沙枣种子并测定其萌发率表明, NaCl对沙枣种子萌发的抑制既有渗透效应又有离子效应, 但是以渗透效应为主; 平罗的沙枣种子表现出更强的耐盐能力, 是因为它有更高的抗渗透胁迫能力。

关键词：沙枣; NaCl; 种子萌发; 离子效应; 渗透效应

收稿：2015-07-01   修定：2015-10-08

资助：国家重点基础研究发展计划(2012CB114201)、国家自然科学基金(31400239)和山东省科技发展计划项目(2013-GNC11310)。

Comparative Study on Seed Germination Characteristics of Elaeagnus angustifolia from Different Areas of Ningxia Province under NaCl Stress

LIN Jing¹, LIU Yan¹, LI Jin-Sheng², LIU Xiao-Juan¹, WANG Yu-Peng¹, WANG Bao-Shan¹, CHEN Min^1,*

¹Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China; ²Dongying Jinghua Modern Forestry Development Limited Company, Dongying, Shandong 257000, China

Corresponding author: CHEN Min; E-mail: chenminrundong@126.com; Tel: 0531-86180745

Abstract:

Elaeagnus angustifolia seeds collected from Pingluo and Zhongning Counties of Ningxia Province were treated with different concentrations of NaCl (0, 0.3%, 0.6%, 0.9% and 1.2%, m/V) of Hoagland solution (1/5, V/V), in order to examine their response characteristics to NaCl strees and possible reasons. The results showed that germination rate of E. angustifolia seeds from both counties were decreased with the increase of NaCl concentration, and E. angustifolia seeds from Pingluo showed stronger salt resistance than those from Zhongning. Evans blue staining and membrane permeability test revealed that the plasma membrane permeability of E. angustifolia seed embryo increased with the increase of NaCl concentration. That was NaCl stress destroyed selective permeability of the seed embryo, resulting in increase of membrane permeability and of intracellular electrolyte leakage, damaging the cell homeostasis. E. angustifolia seeds from both counties were treated with LiCl solution and isosmotic mannitol solution to determine germination rate. The results ndicated that NaCl-induced decrease of E. angustifolia seed germination was mainly due to osmotic stress, and E. angustifolia seeds from Pingluo exhibited stronger salt resistance because of its high capability in resistance to osmotic stress compared with the seeds from Zhongning.

Key words: Elaeagnus angustifolia; NaCl; seed germination; ionic effect; osmotic effect